Molecular study of CTX-M-1, CTX-M2, CTX-M9, mcr-1, NDM-1 genes among Klebsiella pneumonia isolates from hospitalized patients in Taleghani hospital; Tehran, Iran
- سال انتشار: 1398
- محل انتشار: بیستمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM20_071
- زبان مقاله: انگلیسی
- تعداد مشاهده: 485
نویسندگان
Dept. of Microbiology, Faculty of Basic Sciences, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
Dept. of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Dept. of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Introduction and Objectives: Klebsiella pneumonia, is a major cause of hospital infections and the prevalence of drug resistance among them is increasing globaly. The aim of this study was molecular evaluation of CTX-M-1, CTX-M-2, CTX-M-9, mcr-1, NDM-1 genes among Klebsiella pneumonia isolates from hospitalized patients in Taleghani hospital. Materials and methods: Klebsiella pneumonia isolates from patients who referred to Taleghani hospital were collected during2016-2017.All were confirmed by standard bacteriologic tests. Antimicrobial susceptibility test (AST) was done by Kirby-Bauer method based on CLSI protocol. After DNA extraction, frequency of selected resistance genes was determined by PCR method and further sequencing. Statistical analysis was done by SPSS software. Results: among 70 isolates of Klebsiella pneumonia, the highest resistance were against: Ampicillin74%, Cefpodoxime65%, Ceftriaxone 57%, and 55.6% to Aztreonam. The most sensitivity was to Fosfomycin/Trometamol 86.5%, Amikacin, Imipenem, Doripenem, Meropenem, Ertapenem 71.6%. PCR results showed that 71% of the isolates had CTX-M-1 and 31% CTX-M-2, 23% CTX-M-9, 1.4% NDM-1. All were negative for mcr-1gene. Conclusion: with the release of new information on drug resistance and the detection of CTX-M 1,2, 9, mcr-1 and NDM-1 genes in Klebsiella pneumoniae isolates, doing molecular tests are recommended which may help physicians to prescribe the best and most appropriate antibiotic.کلیدواژه ها
Klebsiella pneumoniae, mcr-1, antibiotic resistance, CTX-M gene, NDM-1مقالات مرتبط جدید
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