Antibiotics resistance in isolated Salmonella from Urmia’s poultry farms

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 398

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شناسه ملی سند علمی:

MEDISM20_060

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Introduction and Objectives: Salmonella is a genus bacillus gram-negative bacteria of the Enterobacteriaceae family. Salmonella is the causative agent of bacillary white diarrhea and fowl typhoid in poultry. Materials and Methods: At this study sampling was done from 150 cases of 500 broilers was referred to Urmia university veterinary clinic; and nine of them was infected with Salmonella. After necropsy, sampling was done from broilers’ liver, bile sac, and colon components. after 18h incubation in Selenite F Broth medium, we have grown bacterias in Brilliant Green Agar (BGA) medium and separate negative lactose colonies; we did IMViC and TSI testes on negative lactose colonies for definitive detection. Antibiotics were used as a plate and it was included common antibiotics used. For antibiotic sensitivity test (antibiogram) we grown separated Salmonella’s colonies in Muller-Hinton Agar (M-H Agar) medium; after 24h incubation, we put antibiotic plates in medium and then incubate it for 24h. Results: We recognize that enrofloxacin and ampicillin are the most powerful and effective antibiotics against salmonellosis and all of the samples had resistance against tylosin and tiamulin. Conclusion: overuse and unnecessary use of antibiotics are one of the main reason for antibiotic resistance and according to this article the best antibiotics for against salmonellosis are enrofloxacin, ampicillin, flumequine, and neomycin.

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نویسندگان

Siamak Mollazade

D.V.M., Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Malahat Ahmadi

Professor, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Alireza Talebi

Associate Professor, Department of Midwifery and poultry, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Amir Tukmechi

Associate Professor, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Mahdi Babaei

D.V.M. student, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran