Hepatoprotective effects of biochanin A against acetaminophen induced hepatotoxicity in mice

Background: Acetaminophen (APAP) adverse impacts on the liver as a vital body organ have been demonstrated by the induction of oxidative stress.Objective: The objective of this study was to examine the hepatoprotective effect of biochanin A (BA) in a mice model of APAP -induced hepatotoxicity.Materials and Methods: Animals were divided into six groups. Animal groups included control, APAP, BA 40 mg/kg and three groups which received BA at doses of 10, 20 and 40 mg kg just before and 2 and 4 hours after APAP, respectively. Liver tissue level of malodialdehyde (MDA) and glutathione (GSH) and the activities of superoxide dismutase (SOD) and catalase (CAT) were measured. Histological analysis and measurement of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were performed.Results: APAP-induced hepatotoxicity was manifested by an increase in the serum activity levels of ALT, AST, ALP as well as acinar hepatic necrosis, inflammation and fatty degeneration. APAP decreased hepatic SOD and CAT activity and GSH level and increased in MDA level. BA at the dose 20 mg/kg prevented increase in serum activity levels of ALT, AST. BA 20 mg/kg increased the activity level of hepatic SOD and CAT, and normalized the hepatic histo-architecture. BA decreased hepatic MDA level and had no significant effect on the level of glutathione in APAP-exposed animal.Conclusion: The results indicate BA protected the liver from APAP-induced injury. Prote

کلیدواژه ها:

Acetaminophen ، Oxidative stress ، Biochanin A ، Hepatoprotective ، Mice