Evaluation effect of cinnamaldehyde on phosphoine-induced toxicity in vitro

Purpose: Phosphine (PH3), from hydrolysis of metal phosphides, is an important insecticide (aluminum phosphide) and rodenticide (zinc phosphide) and is considered genotoxic and cytotoxic in mammals. This study was designed to evaluate the protective effects of cinnamaldehyde on the toxicity and oxidative damage of Phosphine in HepG2cell line. Methods: HepG2 cells were exposed to 12.5 to 50 μM concentrations of cinamaldehyde and after 24 hours, phosphine (1mM) was added to the cells and cell viability was measured using MTT assay after 6 hours of exposure. In order to measure the amount of reactive oxygen species, similar procedure was performed using the fluorometric method. In addition, the apoptosis of the cinamaldehyde-treated HepG2 cells against phosphine (1mM) was also evaluated using the PI method. Finally for evaluating the cytotoxicity effect of phosphine, the amount of reduced gluthatione (GSH) was examined. Results: After 6 hours exposure to phosphine, cinamaldehyde could reduce the phosphine- induced cell toxicity and showed significant difference in comparison with Phosphine group. Cinamaldehyde also increased intracellular reactive oxygen species (ROS) in cells and ROS played an important role in phosphine cytotoxicity. Inaddition, Cinnamaldehyde at all concentration plus phosphine reduced the cell apoptosis and increased the level of GSH compared to phosphine group. Conclusion: Based on the results and cinnamaldehyde antioxidants properties, this substance can be considered as a promising compound in the treatment of Phosphine-induced toxicity.

کلیدواژه ها:

Cinnamaldehyde ، Phosphine ، oxidative stress ، apoptosis