Frequency of polymorphisms of CYP2C9 and VKORG1 genes in warfarin users

Introduction: Warfarin is usually prescribed for preventing thrombosis especially in patients with cardiovascular disease. Different drug-responses are observed among patients. Some genetic variations have been described to be involved responses of individuals to drug. In this study, the prevalence of common variants of CYP2C9 and VKORG1 genes is investigated.Materials and Methods: A total of 58 people with cardiovascular disease who treated by warfarin were recruited in this study. Clinical features and the history of drug usage were documented. DNA was extracted from peripheral blood samples. PCR-RFLP was performed for detecting common variants.Results: The mean age of patients was 41 years, ranging from 3 years to 80 years. The CYP2C9 variant gene was observed in 27 individuals. The heterozygous variant had a high frequency. The VKORG1 variant gene was observed in 22 individuals. It was found homozygously in 12 patients.Discussion and Conclusion: Environmental and genetic factors may have a significant effect on treatment; therefore, the study of genetic variations could be helpful to determine the dose of warfarin.

کلیدواژه ها:

Warfarin-CYP2C9-VKORG1-Genotype-PCR-RFLP