Use of 3D culture for studing effect of statins on prostate cancer cell line

Prostate cancer (PCa) is one of the most common malignancies and the second leading cause of cancer-related death in men . Current treatments for PCa including tumor resection with radiotherapy, chemotherapy, and drug therapy are not successful in cancer suppression and decrease of deaths.Recent studies have shown that cholesterol is an emerging clinically relevant therapeutic target in PCa patients. Importantly , high circulating cholesterol level have been shown to increase the risk of overall aggressive PCa. Statins are commonly used in the treatment of hypercholesterolemia to inhibit 3-hydroxy 3-methylglutaryl coenzyme A reductase in mevalonate pathwy and thus prevent the synthesis of cholesterol precursors,such as farnesyl pyrophosphate and geranylgeranyl pyrophosphate.Preclinical studies have reported that statins potentiate antitumor effects.in other side,3D culture as a good model for studing of effects of drugs on cancer.The main aim of the present research is to achieve therapeutic effect of Rosuvastatin on PCa . therefore, cell line-PC3- was cultured in 3D culture and was treated with different concentration of Rosuvastatin and cell survival (IC50) was determined using the methyl-thiazolyl-tetrazolium ( MTT ) assay. Our Finding suggestes rosuvastatin causes growth inhibition in 3D culture in PC3 cell line.

کلیدواژه ها:

3D culture ، prostate cancer ، statin