Promoter analysis of some genes responding to H5N1 virus in chicken

Transcriptionalregulation isknown as a complicated process involving many different proteins, which some of them bind in a specific-DNA sequence harbored within a promoter region of a gene. In this respect, one of the crucial challenges in biotechnology is the recognition of promoter and its regulatory elements. In this study, we isolated the promoter region of several co-expressed genes responding to the H5N1 virus in chicken. Then, cis/trans regulatory elements were identified using TRANSFAC and oPOSSUM databases. According to obtained results, a plethora of overrepresented motifs sharing at most promoter region of these genes were discovered. Among these, however, some ofcis/trans elementswere previously studied and confirmed in term of response to influenza virus. MYB and IRF are well-known elements regulating the expression of particular gene so as to strengthen of innate immune system. Moreover, these specified overrepresented motifs were scattered across the promoters. In conclusion, recognition and understanding of these specific motifs may shed some light regarding to transcription factors response related to influenza infection in chickens, andhas implications for strategies targeting the innate immune system for boosting resistance to avian influenza.

کلیدواژه ها:

Promoter ، Cis-regulatory Elements ، Influenza ، H5N1