A rapid, Simple Genomic DNA Extraction from Yeasts for Large Scale Genetic Analyses
- سال انتشار: 1397
- محل انتشار: سومین کنگره بین المللی و پانزدهمین کنگره ملی ژنتیک ایران
- کد COI اختصاصی: CIGS15_307
- زبان مقاله: انگلیسی
- تعداد مشاهده: 551
نویسندگان
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box ۳۳۵۳۵۱۱۱, Tehran, Iran
چکیده
DNA extraction methods usually rely on time-consuming procedures that may involve handling hazardous compounds or using expensive kit. Here, we have developed a rapid genomic DNA extraction technique from yeasts and applied it for amplification of genomic DNA using gene specific primers. DNA extraction was evaluated at different stages from initial step to final pure DNA. In addition to final pure DNA of each sample which runs on an agarose gel to check the quantity and efficiency of whole yield, the extracts at different stages of extraction procedure were qualitatively examined and evaluated using gene amplification in a PCR reaction for minimal time consumption and lack of PCR inhibitors. The results showed that the earliest step for successful gene amplification was immediate after one hour incubation in 65 oC with extraction buffer. The obtained DNA was validated with different sets of primers for DNA amplification. The PCR products from each sliced bands were purified and sequenced. This evaluation showed that the presented method is a reliable, rapid and simple procedure with minimal equipment and resources requirements which is also environmentally friendly in absence of toxic chemicals for a cost efficient DNA extraction especially for large number of samples in less than one hour for sufficient genomic DNA for different purposes from yeasts for large scale genetic analyses including downstream PCR-based genetic analysis.کلیدواژه ها
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.