Bioinformatics Identification of the Binding Site of Transcription Factors in some Candidate Genes in cow

The aim of the present study was to investigate the expression of genes and transcription factors associated with the replacement stage and before replacing the embryo in the endometrium of the cattle. The expression of the identified genes in the endometrial tissue of the cow species was investigated and the transcriptional regulation network of these genes in the endometrium tissue was examined by promoter analysis. Finally, 13 genes with high expression and known function in two stages of pregnancy (embryo replacement and before it) were selected as candidate genes in the immunization response process stages for promoter analysis. The binding sites of transcription factors on the promoters of these genes are used to better understand the regulatory network of the genes in question. Investigation of the regulatory network involved in the intended stages has led to the identification of several transcription factors (TFs), including TFs that were previously not known as involved factors in these stages.In order to analyze the changes between the expression of the microarray data used the R software and the Affy package, also the GO analysis to identify the biological stages associated with each gene. To identify the binding sites were used the GENOMATIX package. The results show that new known factors may have a regulatory role in the early stages of conception. Also, identification of these factors on the promoter regions of both expression and function genes, and along with the factors confirmed in this section, reinforces their probability of being functional.

کلیدواژه ها:

Promoter analysis ، endometrium ، immune response ، embryonic abortion ، transcription factors.