Spatial and Temporal Three Dimensional Scaffold systems Induces Programming of Stem Cells Differentiation and Tissue Regeneration

Nanofibrous architecture mimics natural extracellular matrix (ECM) and dictate cell proliferation and differentiation to promote tissue regeneration. Additionally, synergistic methods that compartmentalize biological payloads can be achieved by partitioning within polymers. This study examines fabrication techniques and mechanisms involved for osteoblast cell adhesion and proliferation for tissue regeneration. Previously, we have demonstrated that nanofibrous scaffold system promotes mesenchymal stem cells differentiation and bone regeneration. In the current study, we generated 3D nanofibrous scaffolds using electrospinning technique with Polycaprolactone (PCL). Small molecule (GSK3 inhibitor), morphogens (growth factors) or natural antioxidants (AX) were incorporated in matrices. Characterization and payload release studies were performed. Cellular and molecular studies in mesenchymal stem cells or pre-osteoblast cells were examined.SEM reveals the architecture of the scaffolds effectively mimic spatial features of ECM, and the cells adhered to the fiber matrix. FTIR confirmed solvent was completely evaporated during process. Biochemical assays demonstrated that biomolecules have incorporated and released in a controlled manner. The biomimetic nanofibrous matrix significantly induces cells adhesion, proliferation and differentiation. Our study provided evidence that a delivery of morphogens or small molecules in combination with scaffold system induce the process of cell differentiation and tissue regeneration both in-vitro and in-vivo respectively.The generation of these sophisticated, spatiotemporal scaffold systems serve as a platform for modular clinical translational systems capable of directing stem cell responses for tissue regeneration.

کلیدواژه ها:

Nanofiber ، small molecule ، stem cells ، pre-osteoblast ، bone regeneration