Therapeutic potential Mesenchymal Stem Cells derived from Human Wharton s Jelly in Alzheimer Rat Models

Background and Aim : Alzheimer s disease (AD) is the most common cause of dementia in adulthood, which is followed by cognitive impairment and behavioral deficits. Today, mesenchymal stem cell (MSC) therapy is a good platform for the development of regenerative medicine in neurodegenerative disorders including AD. The aim of this study is to assess the potential of the human umbilical cord-derived mesenchymal stem cells on progression of AD especially in Rat brain s hippocampus region and also evaluation of the NGF and BDNF and apoptotic genes expression in comparison with model.Methods : After Establishing Alzheimer rat model, then intravenous injecting the MSCs were isolated from human umbilical cord, respectively. MSCs were confirmed by flow cytometry (FC) and immunocytochemistry (ICC), reverse transcription polymerase chain reaction (Real time PCR), were used to assess the expression of genes involved in Neurogenesis, such as the NGF and BDNF genes, as well as the genes involved in the apoptotic process Such as Bcl2, Caspase3, Bax. Level of dark and light cells were assessed by Nissl stainingResults : FC and ICC showed that More than 80% of the cells expressed mesenchymal markers. These cells also differentiated into both bone and fatty groups. In the treatment group, apoptosis genes expression decreased and NGF and BDNF expression increased. Human umbilical cord stem cells have improved effects on AD rats.Conclusion : WJ-MSCs are readily isolated from the umbilical cord and proliferate rapidly in vitro. the application of MSCs can significantly improve the learning and memory. the expression of NGF and BDNF genes increased and apoptosis gene expression decreased. STZ injection into the brain ventricles causes severe learning and memory impairment.

کلیدواژه ها:

Alzheimer’s model ، mesenchymal stem cells ، apoptotic markers ، BDNF ، NGF ، Wharton’s jelly