Maryam Sadat Jalali - Student of Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Ghasem Saki - Department of Anatomical Sciences, Cellular & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences
Alireza Sarkaki - Dept. of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz- Iran
Yaghoob Farbood - Dept. of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz- Iran
Saeed Azandeh - Department of Anatomical Sciences, Cellular & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences

Background and Aim : Parkinson’s disease (PD) is a widespread neurodegenerative disorder with the oxidation of dopamine (DA), generates reactive oxygen species (ROS) and an imbalanced production of ROS, leading to oxidative stress (OS) and neuronal deathv .Recently the stem cell transplantation (SCT) has become an effective treatment for this disease and human Wharton s jelly-derived MSCs (hWJ-MSCs) have shown more beneficial effects to expand neural cells. In the present study we examined the effects of hWJ-MSCs on on locomotion, pallidal local EEG, and its frequency bands power in a rat model of PD induced by 6-hydroxidopamine (6-OHDA).Methods : PD was induced by injection of 6-OHDA (16 μg/2 μl) into the right medial forebrain bundle (MFB). Male Wistar rats were divided into 5 groups: Group 1 (control) : received saline Normal. Group 2(PD) [6-OHDA)], Group3 (PD+C): after lesion received HWJ-MSCs (1*106 Cells) i.v, Group 4 (PD+C+drug):after lesion treated by received HWJ-MSCs (1*106 Cells) and (10 mg/kg/levodapa, 30mg/kg/carbiodapa PO, for 60 days after PD induction, Group 5(PD +drug) (after lesion received 10 mg/kg/levodapa, 30mg/kg/carbiodapa PO). Motor activity was assessed by stride length, rotarod, muscle stiffness(Morpurgo), bar test and cylinder tests. Pallidal local EEG was recorded in freely moving rats.Results : MFB lesion caused significant reduction of stride-length (P<0.001), bar decent latency (P<0.001) and frequency bands power of pallidal EEG (P<0.001) and also increased the contralateral rotations (P<0.001) latancy in bar and morprogo tests and also score of the cylinder test (P<0.001). hWJ-MSCs with Levodopa/Carbidopa significantly restored all above parametersConclusion : Although the use of Levodopa/Carbidopa or stem cells can improve the motor movements in PD but the best results were found in PD+cell+Drug group and it can be suggested that the use of drugs such as L-Dopa / Carbidopa and also hWJ-MSCs together can be a good treatment strategy in PD patients.

Parkinson s disease, Stem cells, Motor disorder, Electroencephalogram(EEG)