Effects of thymoquinone on motor function and the dentate gyrus electrophysiological activity in an animal model of hepatic encephalopathy

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 352

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شناسه ملی سند علمی:

NSCMED08_231

تاریخ نمایه سازی: 15 دی 1398

چکیده مقاله:

Background and Aim : Hepatic Encephalopathy (HE) is a neurological complication of liver failure, which can lead to psychomotor dysfunctions, impaired memory, shortened attention that may finally lead to coma and death. A toxin model of hepatic encephalopathy was selected using thioacetamide (TAA). This model has been used to clarify changes in the functions of the CNS in HE. Thymoquinone (TQ), the main active constituent of Nigella Saliva seeds, is reported to have strong free radical scavenger, antioxidant and anti-inflammatory properties in different animal tissue models. The purpose of the present study was to investigate motor coordination behavior and the dentate gyrus (DG) electrophysiological activity on rats with TAA-induced liver damage and hepatic encephalopathyMethods : Male Wistar rats were divided into 5 groups randomly: 1) Control; received normal saline. 2) HE; received TAA (200 mg/kg). 3) HE + TQ5. 4) HE + TQ10. 5) HE + TQ 20 (mg/kg, i.p.) for 7 days after HE induction. In order to induce animal model of HE, TAA (200 mg/kg) were injected intraperitoneal once every two days for consecutive 14 days. Rotarod tests performed to evaluate the motor activity and coordination. Neuronal single unit recording was assessed from hippocampal DG. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test and changes with p value less than 0.05 assign as significant differenceResults : Data showed HE induction impairment of motor coordination rats as compared with control rats, while motor function were significantly improved in TQ treatment groups when compared with those of HE rats in a dose dependent manner (P < 0.001). The results showed that the average number of spikes/bin was significantly decreased in the HE rats compared with the control group (p < 0.001) and injections of TQ lead to a significant increase in the spike rate compared with the HE group (p < 0.001 and p < 0.01)Conclusion : Our results showed that HE induced impairment in motor function and electrophysiological damages in rats. Administration of TQ after HE induction reveals therapeutic potential to motor coordination activity and electrophysiological dysfunction which is most likely related at least to its antioxidative and free radical scavenging actions.

نویسندگان

Somayeh Hajipour

Physiology Research Center. Ahvaz Jundishapur University of Medical Sciences

Alireza Sarkaki

Dept. of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences

Yaghoub Farbood

Dept. of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences

Mahin Dianat

Dept. of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences

Mohammad Rashno

Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences

Leya sadat Khorsandi

Department of Anatomical Sciences, Cellular & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences