Stress-induced memory impairment: Involvement of hippocampal miR-1 and CREB/BDNF signaling pathway

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 326

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شناسه ملی سند علمی:

NSCMED08_160

تاریخ نمایه سازی: 15 دی 1398

چکیده مقاله:

Background and Aim : Pre-clinical and Clinical studies have shown that physiological or psychological stressors via activating the HPA axis led to the release of corticosterone or cortisol in rodents and humans, respectively. Crossing of glucocorticoids from blood brain barrier and their binding to the mineralocorticoid and glucocorticoid receptors influence a variety of physiological functions including memory consolidation and retrieval. Both coping with stressful stimuli and forming of new memory were suggested to change protein synthesis and degradation in the brain. MicroRNAs (miRs), small non-coding RNAs, have a modulatory role in the post-transcriptional gene silencing via binding to 3 UTR of mRNAs to regulate different cellular processes including memory-induced protein synthesis. Considering these, the aim of the present study was to investigate the possible involvement of hippocampal mir-1 as well as CREB/BDNF signaling pathway in stress induced memory impairment.Methods : A step-through passive avoidance task was used to assess memory performance in male Wistar rats (200-220 g). An elevated platform (EP) apparatus was also used to induce stress. The alterations in the p-CREB/CREB ratio, and the levels of BDNF in the hippocampus and also miR-1 expression were examined by Western blot analysis and real time PCR, respectively.Results : The results showed that post-training exposure to acute stress induced amnesia associated with up-regulation of miR-1 in hippocampus. Also, the exposure to the acute EP stress reduced the hippocampal p-CREB/CREB ratio and the levels of BDNF.Conclusion : Taken together, these findings suggest that the exposure to the acute stress induces memory impairment possibly via the attenuating of the p-CREB/CREB ratio and BDNF levels in the hippocampus of the rats. Our results shown that miR-1 up-regulation possibly involve in down-regulation of p-CREB/CREB ratio and BDNF levels in the hippocampus.

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نویسندگان

Mehdi Eivani

Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Sakineh Aljanpour

Department of Biology, Faculty of Science, Gonbad Kavous University, Gonbad Kavous, Iran

Ehsan Arefian

Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Ameneh Rezayof

Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran