EFFECTS OF BDNF RECEPTOR ANTAGONIST ON THE EXTINCTIONAND REINSTATEMENT OF MORPHINEINDUCED- CONDITIONED PLACE PREFERENCE AND MPFC BDNF LEVELS AND APOPTOTIC CELLS IN RATS

  • سال انتشار: 1398
  • محل انتشار: سیزدهمین کنگره بین المللی دانش اعتیاد
  • کد COI اختصاصی: KAMED13_154
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 438
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نویسندگان

Marzieh Joneidi

Resserch Center and Department of Physiology, School of Medicine ,Semnan University of Medical Sciences, Semnan, Iran

Hossein Miladi-Gorji

Resserch Center and Department of Physiology, School of Medicine ,Semnan University of Medical Sciences, Semnan, Iran

abbas Ali Vafaei

Resserch Center and Department of Physiology, School of Medicine ,Semnan University of Medical Sciences, Semnan, Iran

Hamidreza Sameni

Department of Histoloy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

Ahmad Reza Bandegi

Department of Biochemistry, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

Behpoor Yousefi

Department of Anatomical , School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

چکیده

Background and Aim : It is likely that BDNF is necessary to plasticity induced by opiates in the dopaminergic neurons for the morphine-induced conditioned place preference. Therefore، this study examined the effects of BDNF receptor antagonist (ANA-12) on the mpFC and Extinction and reinstatement of morphine-induced conditioned place preference (CPP) mPFC BDNF levels and apoptotic cells in rats.Methods : In this study،48 adult male Wistar rat (200-250 g) wera used. The rewarding properties of morphine were investigated using a CPP paradigm consisted of 5-day schedule with three distinct phases: pre-conditioning, conditioning and post- conditioning. Bilateral guide cannulas were stereotaxically implanted into the central region of the mPFC in rats. Rats were then trained in the CPP after the recovery period. To assess،ANA-12To assess the effect of ANA-12 on extinction،after a conditioning test day an ANA-12 is injected for three days and after 5 daysextintion،test Post Conditioninand toreinstatement assess 5day, and the day before the test reinstatement of the rat a test extinction was performed to ensure the extinction period. On the day ofreinstatement ANA-12, it was injected in the frontal cortex area and 15 minutes later a neutral dose of morphine was injected intraperitoneally.Then, the levels of BDNF and apoptotic cellsof the mPFC were assessed at the end of each treatment phase using ELISA and TUNEL methods respectively.Results : The results of this study indicated that intra-mPFC injections of ANA-12 significantly attenuated the extinction of morphine-induced CPP.but there was no significant effect of BDNF onthe median prefrontal cortex.But in the ANA-12 Reinstatement group, there is no significant in the preferences of the conditional place.Also, the level of BDNF in the receiving group of ANA-12did not significant difference with receiving vehicle group.As well as antagonizing the BDNF on motor activity rat is ineffective.Conclusion : This study demonstrated that central administration of ANA-12 in the mPFC attenuates the o fextinction and reinstatement morphine-induced CPP, via a TrkB-mediated mechanism

کلیدواژه ها

Morphine, mPFC, BDNF levels,apoptosis

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