Yadollah Bahrami - Young Researchers Club and Elites, Khorasgan Branch, Islamic Azad University, Khorasgan, Iran
Sajad Bahrami - Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
Elham Rezvannejad - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
Seyed Azim Mousavizadeh - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Booroola gene is one of the major genes in the enhancement of ovulation rate and could be an attractive candidate gene for ovulation rate in sheep. Molecular technologies have been widely used for discovery of mutations in animal species. These mutations have major effects on the important economic traits of sheep and goats. This study was conducted to identify the mutation in FecXG region of exon 2 of BMP15 gene which is associated with twining using polymerase chain reaction-restriction fragment length polymorphism method in Hisari Sheep. For this purpose, blood samples were obtained from 110 sheep. DNA was extracted using the salting out method and quantity and quality properties of DNA were determined using electrophoresis gel. Afterward, a 141 bp fragment, which contains the given polymorphic site of BMP15 gene, was amplified by the specific primers. Restriction enzyme (HinfI G/ANTC) cuts the wild-type allele and as a result of digestion, 112 and 29 bp fragments produced. Mutant type allele is not cut because of the removal of the intended cutting site. All the samples showed the similar monomorphic of genotype (+/+) (wild genotype). Results of this study indicate that the investigated polymorphism is not present in the hisari sheep

polymorphism, BMP15gene, Hisari sheep, Twining, PCR-RFLP