Correlation between Expression of hsa-miR-490-5p and NFAT5 in Peripheral Blood Mononuclear Cell Obtained from Breast Cancer Patients
- سال انتشار: 1398
- محل انتشار: مجله منابع ژنتیک، دوره: 5، شماره: 1
- کد COI اختصاصی: JR_SGR-5-1_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 597
نویسندگان
Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
چکیده
Breast cancer is a complex genetic disease that has an average annual incidence of two million people and the second leading cause of death among women all over the world. Micro-RNAs are consistently reported to regulate gene expression in all cancers. The present study, the correlation between the expression of hsa-miR-490-5p and nuclear factor of activated T-cells 5 (NFAT5) in breast cancer were investigated. NFAT5 nuclear accumulation occurs regardless of Wnt/β-catenin activated signaling in a substantial portion of breast cancer. The analysis of prediction target and dual-luciferase reporter assays supported that hsa-miR-490-5p directly targeted NFAT5 and suppressed the expression of NFAT5. In a cross-sectional comparative study, peripheral blood samples were collected from 30 subjects with breast cancer and 30 healthy individuals as a control group. Total mRNA was extracted from peripheral blood mononuclear cells (PBMCs) and cDNA was synthesized to study the NFAT5 and hsa-miR-490-5p gene expression variations by real-time PCR. A significant decrease was observed in gene expression and sera concentration of NFAT5, hsa-miR-490-5p in PBMCs of breast cancer patients. The obtained results indicated that hsa-miR-490-5p acts as oncomir in serum by targeting NFAT5 direct (p< 0.05).کلیدواژه ها
hsa-miR-490-5p, NFAT5, Breast cancer, PBMCsاطلاعات بیشتر در مورد COI
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