Isolation and identification of Asaia sp. in Aedes aegypti spp. Mosquitoes
- سال انتشار: 1398
- محل انتشار: دومین کنگره بین المللی بیماریهای منتقله بوسیله ناقلین و تغییرات آب و هوایی و چهارمین کنگره ملی حشره شناسی پزشکی ایران
- کد COI اختصاصی: DCME02_268
- زبان مقاله: انگلیسی
- تعداد مشاهده: 469
نویسندگان
Cellular and Molecular biology MSCs, Department of Medical Entomology, School of Health, Islamic azad University of Medical Sciences, Tehran, Iran
Associated professor, Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
Medical entomology MSCs, Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
Laboratory science BSc, Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
Laboratory science BSc, Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
Professor, Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran.
چکیده
Background: In recent years, the genus Asaia has been isolated from different insects such as: Anopheles, Culex and Aedes. This bacterium has unique features such as presence in different organs of mosquitoes (midgut, salivary glands and reproductive organs) of female and male mosquitoes and vertical and horizontal transmission. These specifications lead to the possibility of introducing Asaia as a robust candidate for different control of vector borne diseases via paratransgenesis technology. Several studies have been performed on the microbiota of Aedes mosquitoes in Iran and the Middle East to find a suitable candidate for controlling the malaria based on paratransgenesis approaches. The present study is the first report of isolation and characterization of the genus Asaia within Aedes aegypti species which originated from southeast and east of Iran.Objectives: The main purpose of this study is isolation, biochemical and molecular characterization of the genus Asaia within Aedes aegypti species originated from different part of the Iran. Materials and Methods: Mosquitoes originated from field-collected and laboratory-reared colonies of Aedes aegypti species. Adult mosquitoes were anesthetized; their midguts were isolated by dissection, followed by grinding the midgut contents which were then cultured in enrichment broth media and later in CaCO3 agar plates separately. Morphological, biochemical and physiological characterization were carried out after the appearance of colonies. For molecular confirmation, selected colonies were cultured, their DNAs were extracted and PCR was performed on the 16S ribosomal RNA.Results: In this study, morphological, biochemical, physiological and molecular results indicated that all isolates are members of the genus Asaia bacteria. Bacterium were identifiedin the middle midgut of the Aedes aegypti mosquitoes by using the culture dependent method and sequencing the 16s rRNA gene.Conclusion: Our findings show that Asaia bacteria are present in both insectary-reared colonies and field-collected mosquitoes and can be isolated by simple and specific methods. Furthermore, with respect to the fact that we isolated Asaia within the Aedes aegypti specimens from distinct climatic and zoogeographical regions, it is promising and may be concluded that species of this genus can tolerate the complicated environmental conditions of the vector-borne diseases endemic regions. Therefore, it can be considered as a promising target in paratransgenesis and vector control programs.کلیدواژه ها
Paratransgenesis, Asaia, Bacteria, Vector-borne diseases, Vector control paratransgenic, Microbiota, Aedesاطلاعات بیشتر در مورد COI
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