Agglutinin activity in the gut of PHLEBOTOMUS PAPATASI
- سال انتشار: 1398
- محل انتشار: دومین کنگره بین المللی بیماریهای منتقله بوسیله ناقلین و تغییرات آب و هوایی و چهارمین کنگره ملی حشره شناسی پزشکی ایران
- کد COI اختصاصی: DCME02_167
- زبان مقاله: انگلیسی
- تعداد مشاهده: 387
نویسندگان
Tehran University of Medical Sciences, School of Public Health, Department of medical entomology and vector control
Tehran University of Medical Sciences, School of Public Health, Department of medical entomology and vector control
Tehran University of Medical Sciences, School of Public Health, Department of medical entomology and vector control
چکیده
Background: Zoonotic Cutaneous Leishmaniasis (ZCL) is a neglected tropical disease prevailed in many rural areas of 17 out of 31 provinces in Iran. The main vector of the disease is Phlebotomus papatasi. In the present study, we investigate, the inhibitory role of some carbohydrates on lectin activities of Ph. papatasi Objectives: The candidate carbohydrates found some species independent, so study on different Leishmania/sandfly species recommendedMaterials and Methods: The first generation of wild captured sandflies was colonized and then the females with 3 to 5 days-old (sugar-fed by sucrose 20%) were dissected individually in phosphate-buffered saline (PBS) at pH 7.2. The whole proteins were extracted from midgut of 600 females Ph. Papatasi. Serial dilutions of the gut extraction were prepared and haemagglutination tests conducted against human, rat and Balb/C red blood cells. Furthermore, inhibition assays were performed against lectin actives using fructose, fucose, galactose, lactose, glucose, arabinose, D-mannose, N- acetyl-D- galactoseamineResults: The gut protein of Ph. papatasi showed high agglutinin activity against rate RBC. Moreover, the lectin activity was inhibited highly with arabinose, fucose and mannose.Conclusion: The present of lectin activity in the gut of the sandfly vector may play as a receptors for lieshmania parasites, and some carbohydrate such arabinose may mediate for attachment of the parasite onto the gut surface. Further investigation is carrying on to confirm the role of arbohydrate/lectin interaction for development of lieshmania parasite in this vector.کلیدواژه ها
Lectin, carbohydrates, ph. Papatasi, inhibitorاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.