Molecular identification of Dipylidium caninum in Ctenocephalides canis in a visceral leishmaniasis area of Iran.
- سال انتشار: 1398
- محل انتشار: دومین کنگره بین المللی بیماریهای منتقله بوسیله ناقلین و تغییرات آب و هوایی و چهارمین کنگره ملی حشره شناسی پزشکی ایران
- کد COI اختصاصی: DCME02_076
- زبان مقاله: انگلیسی
- تعداد مشاهده: 577
نویسندگان
Department of Health, Firoozabad Branch, Islamic Azad University, Firoozabad, Iran.
Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background: In the different part of world, Canine have a major role in the transmission of viral parasite. Different species of arthropods such as fleas, ticks, mites and luses inhabit on the body of dogs as ectoparasites. Fleas are important ectoparasites and agent of allergic dermatitis. They serve as intermediate host to for parasitic worms, and transmit distinct pathogens.Objectives: The aim of this study is to survey the infection of Dipylidium caninum in fleas of dogs.Materials and Methods: Overall, 947 fleas were collected using brushing against the hair of dogs during (2014-2015). At first fleas were identified morphologically, then they were preserved in 90% ethanol for molecular identification. the 28S ribosomal RNA gene of Dipylidium caninum was amplified using LIN primers in PCR assay.Results: In the morphological study, three species were identified as follows: Ctenocephalides canis, Pulex irritans and Ctenocephalides felis. The results of PCR assay and sequence analysis indicated that 13 Ct. canis specimens on infected dogs were positive for Dipylidium caninum. also, no infection was observed in P. irritans and Ct. felis (Gene bank code: 2077640).Conclusion: According to our observation, Ct. canis was the dominant species circulating species of parasites including Dipylidium caninum among reservoirsکلیدواژه ها
Dipylidium caninum Flea, Dog, rRNA, visceral leishmaniasis area, Iranمقالات مرتبط جدید
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