Effects of diet-induced obesity on tracheal responsiveness to methacholine, tracheal visfatin level, and lung histological changes in ovalbumin-sensitized female wistar rats
- سال انتشار: 1397
- محل انتشار: کنگره بین المللی بیماری های راه های هوایی و بینابینی ریه
- کد COI اختصاصی: AILDMED01_036
- زبان مقاله: انگلیسی
- تعداد مشاهده: 637
نویسندگان
Ardabil Imam Khomeini Educational and Clinical Hospital, Ardabil University of Medical Sciences, Ardabil, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
چکیده
Introduction: Many studies have shown a close relationship between obesity and asthma severity. In the present study, the effects of diet-induced obesity were examinedon airway responsiveness to methacholine in addition to visfatin level in female Wistar rats’ tracheae after sensitization with ovalbumin. Materials and Methods: The rats were divided into four groups: control with normal diet (ND), ovalbumin (OVA)-sensitized with normal diet (S+ND), high-fat diet (HFD), and OVAsensitizedwith a high-fat diet (S + HFD). The animals were fed for 8 weeks with standard pelts or high-fat diet and then sensitized and challenged with OVA or saline for another 4 weeks. At the end of the study, the tracheae were isolated and assessed for airway responsiveness and visfatin protein levels. Results: Diet-induced obesity groups developed increased weight and obesity indices (p < 0.001). After sensitization with OVA and diet-induced obesity, there were marked leftward shifts in methacholine concentration-response curves in S + HFD group compared to other groups. Also, maximum response was the highest (P< 0.05 to P< 0.001), EC50 was the lowest (P< 0.05 to P< 0.001), and visfatin protein level was the highest (P< 0.05 to P < 0.01) in S + HFD. Conclusion: According to results, diet-induced obesity caused airway hyperresponsiveness to methacholine and enhanced visfatin protein levels in the tracheae of ovalbuminsensitized female rats. Our results suggested that, in obese ovalbumin-sensitizedconditions in female rats, the local production of adipocytokines, such as visfatin, may be increased, resulting in the deterioration of inflammation in lungs. This finding shows a possible mechanism for the altered phenotype in obesityovalbumin sensitization conditions in female rats.کلیدواژه ها
Airway hyperresponsiveness, Asthma, Obesity, Visfatin, Wistar rats.مقالات مرتبط جدید
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