Bio-fertilizer application: molecular and biochemical changes in infected cucumber with Phytophthora melonis

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: فارسی
مشاهده: 465

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شناسه ملی سند علمی:

JR_BIO-8-1_001

تاریخ نمایه سازی: 2 آذر 1398

چکیده مقاله:

In this work, the effects of two commercial bio-fungicides Biosubtil (Bacillus subtilis) and the bio-fertilizer Biophosphorus (Pseudomonas species), was assayed on the disease incidence, changes in the defensive enzyme activity, the total phenolic content and the expression level of some defense-related genes using real-time PCR method. Biochemical and molecular changes were measured at four time point: 24, 48, 72 and 96 hours post inoculation (hpi) of cucumber plantlets with Phytophthora melonis. The disease incidence decreased through applications of both antagonists. The highest disease reduction (60%) was noted for Biosubtil application compared to control plants. Biochemical analysis showed that both bio-fertilizers are able to increase total protein and phenolic compounds in infected cucumber plants. A maximum increase was observed with biophosphorus application at 72hpi. High activity of Peroxidae )PO) and β-1,3-glucanase enzymes were noted for biophosphorus application at 72 hpi while maximum increase of Polyphenol oxidase (PPO) was observed at 96 hpi for this application. Altered transcript levels of various resistance genes including cucumber pathogen-induced 4 (Cupi4), Lipoxygenase (Lox), Phenylalanine ammonia lyase (PAL) and Galactinol synthase (Gal) were recorded in both treatments. A high expression level (9.5 fold) among the treated genes was observed for Lox gene at 72 hpi and an high expression level for other genes was noted at 48 hpi when cucumber plants treated with Biophosporus. The results suggest that both bio-fertilizers are able to enhance disease resistance in cucumber through induce of resistance mechanisms and could be used as a resistance inducer in the greenhouse condition.

نویسندگان

سید کاظم صباغ

هیات علمی دانشگاه یزد

لیلا تناور

Department of Plant Protection, University of Zabol, Zabol, Iran

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  • Abkhoo J, Sabbagh S (2015) Control of Phytophthora melonis damping-off, ...
  • Ahmadzadeh M, Tehrani AS (2009) Evaluation of fluorescent pseudomonads for ...
  • Arfaoui A (2007) Treatment of chickpea with Rhizobium isolates enhances ...
  • Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in ...
  • Blée E (2002) Impact of phyto-oxylipins in plant defense. Trends ...
  • Bradford M (1976) Rapid and sensitive method for the quantitation ...
  • Chen C, Belanger RR, Benhamou N, Paulitz TC (2000) Defense ...
  • Chérif M, Asselin A, Bélanger R (1994) Defense responses induced ...
  • Choi D, Bostock RM, Avdiushko S, Hildebrand DF (1994) Lipid-derived ...
  • Collinge D, Lyngs Jrgensen H (2009) Effects of b-1, 3-glucan ...
  • Collinge DB, Slusarenko AJ (1987) Plant gene expression in response ...
  • Conrath U, Pieterse CM, Mauch-Mani B (2002) Priming in plant–pathogen ...
  • Cowan MM (1999) Plant products as antimicrobial agents. Clinical Microbiology ...
  • Daayf F, Bel-Rhlid R, Bélanger RR (1997) Methyl Ester ofp-Coumaric ...
  • de Ascensao AR, Dubery IA (2003) Soluble and wall-bound phenolics ...
  • Dordas C (2009) Role of nutrients in controlling plant diseases ...
  • Elad Y (2000) Biological control of foliar pathogens by means ...
  • Esmaeili Shirazifard A, Banihashemi Z (2009) The role of Phytophthora ...
  • Expert J, Digat B (1995) Biocontrol of Sclerotinia wilt of ...
  • Feussner I, Wasternack C (2002) The lipoxygenase pathway. Annual Review ...
  • Gupta P, Ravi I, Sharma V (2013)Induction of β-1, 3-glucanase ...
  • Harborne JB (1984) Phenolic compounds. In: In: Harborne JB (ed.), ...
  • Hausbeck MK, Lamour KH (2004) Phytophthora capsici on vegetable crops: ...
  • Ho H (1986) Phytophthora melonis and P. sinensis synonymous with ...
  • Hofmann J, El Ashry AEN, Anwar S, Erban A, Kopka ...
  • Khosrowfar F, Banihashemi Z (2004) Role of weeds on the ...
  • Kim MS (2008) Galactinol is a signaling component of the ...
  • Kloepper JW, Ryu C-M, Zhang S (2004) Induced systemic resistance ...
  • Kloepper JW, Schroth MN (1978) Plant growth promoting rhizobacteria on ...
  • Li Y, Minerdi D, Garibaldi A, Gullino ML (2009) Molecular ...
  • Lifshitz R, Kloepper JW, Kozlowski M, Simonson C, Carlson J, ...
  • Matern U, Grimmig B, Kneusel RE (1995) Plant cell wall ...
  • Meena B, Radhajeyalakshmi R, Vidhyasekaran P, Velazhahan R (2000) Effect ...
  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance Trends ...
  • Moghaddam MRB, Van den Ende W (2012) Sugars and plant ...
  • Nawar H, Kuti J (2003) Phytoalexin synthesis and peroxidase activity ...
  • Nemati M, Navabpour S (2012) Study on quantitative expression pattern ...
  • Nicholson RL, Hammerschmidt R (1992) Phenolic compounds and their role ...
  • Ongena M, Jacques P (2008) Bacillus lipopeptides: versatile weapons for ...
  • Paul D, Sarma Y (2005) Pseudomonas fluorescens mediated systemic resistance ...
  • Phuntumart V, Marro P, Métraux J-P, Sticher L (2006) A ...
  • Porta H, Rocha-Sosa M (2002) Plant lipoxygenases. Physiological and Molecular ...
  • Rahman M, Punja ZK (2005) Biochemistry of ginseng root tissues ...
  • Ramamoorthy V, Raguchander T, Samiyappan R (2002) Induction of defense-related ...
  • Ramjegathesh R, Samiyappan R, Raguchander T, Prabakar K, Saravanakumar D ...
  • Reuveni R, Shimoni M, Crute I (1991) An association between ...
  • Sabbagh S, Roudini M, Panjehkeh N (2017) Systemic resistance induced ...
  • Sabbagh SK, Valizadeh S (2016). Effect of bio-fertilizers on greenhouse ...
  • Shah J (2003) The salicylic acid loop in plant defense ...
  • Shirzad A, Fallahzadeh-Mamaghani V, Pazhouhandeh M (2012) Antagonistic potential of ...
  • Siedow J (1991) Plant lipoxygenase: structure and function. Annual Review ...
  • Silva M, Guerra-Guimarães L, Loureiro A, Nicole M (2008) Involvement ...
  • Simons TJ, Ross A (1970) Enhanced peroxidase activity associated with ...
  • Šukalović V, Veljović‐Jovanović S, Maksimović JD, Maksimović Pajić Z (2010) ...
  • Van Loon L, Bakker P, Pieterse C (1998) Systemic resistance ...
  • Wu Z, Wang XR, Blomquist G (2002) Evaluation of PCR ...
  • Zhang W, Dick W, Hoitink H (1996) Compost-induced systemic acquired ...
  • نمایش کامل مراجع