The inhibitory effect of lentiviral-Let7b on HCV RNA replication
سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 391
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شناسه ملی سند علمی:
ICCM13_017
تاریخ نمایه سازی: 25 آبان 1398
چکیده مقاله:
Background: Treatment and prevention of hepatitis C virus (HCV) infections remain a major challenge for controlling this worldwide health problem controlling this worldwide health problem. Methodes and Matherials: In this study, we investigated the role of let-7b that were overexpressed by lentiviral vector against highly conserved HCV-1 sequences. Since the main target of this microRNA is the NS3 gene,it was amplified, cloned into ptz57R/T cloning vestor and then subcloned into psicheck-2, respectively. In order to doing Luciferase assay test, lentiviral vector-let-7b and moc were transduced into Huh-7.5 as a hepatocyte origine cells and then schicheck-2 constructs were transformed into them. Results: Follwing performance of a luciferase assay, the specific binding of this microRNA to the viral genome was confirmed. Conclusions:This data suggest that let-7b can downregulate of HCV proliferation in Huh-7.5 cell lines.
نویسندگان
Marzieh Jamalidoust
Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Namazi Hospital, Shiraz, Iran
Maryam Shafaati
Department of Microbiology, Jahrom Branch, Islamic Azad university, Jahrom , Iran
Mohammad Kargar
Department of Microbiology, Jahrom Branch, Islamic Azad university, Jahrom , Iran
Maryam Zare
Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Namazi Hospital, Shiraz, Iran