Biochemical Studies of New Schiff Base Ligand and It’s Complex in Water

In the area of bioinorganic chemistry, the Schiff base complexes have been considered as synthetic models for the metal containing sites in metalloproteins and metalloenzymes. Many transition metal ions in living systems can work as enzymes or carriers in a macrocyclic ligand environment. Thus, during the last decade, great attention has been focused on the area of Schiff base complexes [1]. This attention is still growing, so that a considerable research effort is today devoted to the synthesis of new Schiff base complexes with transition and main group metal ions [2]. In this work, UV-Vis, fluorescence and viscometry techniques was used to the investigation of DNA interaction with the synthesized Schiff base ligand and its nickel complex in water. The results revealed hypochromism effects which generally indicate the intercalative binding nature of the interaction. The Kb and Kf were calculated. The binding constants (Kb) for Schiff base ligand and its nickel complex were obtained 9.11 × 104 and 2.46 × 104, respectively.