Combretastatin A-4 (CA-4) Induce P-53 Independent Apoptosis in Glioblastoma Cell Line (U-87MG)
- سال انتشار: 1398
- محل انتشار: سومین همایش بین المللی التهاب سیستم عصبی و سومین فستیوال دانشجویی علوم اعصاب
- کد COI اختصاصی: NIMED03_173
- زبان مقاله: انگلیسی
- تعداد مشاهده: 423
نویسندگان
Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Neurosciences, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Glioblastoma is the most prevalent primary malignant brain tumor. Survival is mostly less than one year, and most patients die within twoyears. Standard therapy includes surgical resection and generally followed by radiotherapy and chemotherapy. CA4 as an antimitotic agent can strongly cause vascular shutdown and cell death in tumors by binding the colchicine binding site of tubulin to block microtubuleassembly. The aim of this stud was to investigate the apoptotic and cytotoxic effect of CA4 in glioblastoma multiforme (U87) cell line. Materials and Methods: Cellular cytotoxicity was measured by rezasurin assay and intracellular active oxygen species were measuredusing DCFDA (2 ‘and 7’-dichloro fluorescein diacetate) assay kit. In addition, apoptotic cells were detected with an Annexin V-FITC early apoptosis staining and cell cycle assay was investigated by propidium iodide (PI). Furthermore, Reatime PCR used to detect the alterationin gene expression of Apoptotic genes. Results: CA4 reduced the proliferation of U87 cells in a concentrationdependent manner. IC50 was 14 and 12.5 μg/mL after 24 and 48 hours, respectively. No significant increase observed in reactive oxygen spices level. Cell cycle distribution analysis showed significant increase in cell population in sub G1 area in concentration-dependent manner. Moreover, Anexin/PI analysis showed an increase in apoptotic cells after treatment by CA4. Significant alteration is not observed in gene expression of Bax, Bcl-2, and P-53. Conclusion: The study show that CA-4 can induce apoptosis in glioblastoma cells which seems it is p53-independent.کلیدواژه ها
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.