A Review on the Future of Protein Analysis in Pathology
- سال انتشار: 1398
- محل انتشار: بیست و یکمین همایش سالانه و چهارمین همایش بین المللی آسیب شناسی و طب آزمایشگاه و هشتمین همایش بین المللی شاخه ایرانی آکادمی بین المللی پاتولوژی
- کد COI اختصاصی: ACPLMED21_074
- زبان مقاله: انگلیسی
- تعداد مشاهده: 564
نویسندگان
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
the use of microscopy images for clinical diagnosis rests at the core of the pathologist s profession. In the last few years, protein analysis methods such as laser-based biological Mass Spectrometry (MS) and immunohistochemistry (IHC) has made substantial advances in countless and very diverse directions. Many techniques have been developed for molecular protein analysis. Especially, matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS). This research aims to focus on the workflow, advantages, and disadvantages of both MALDI IMS and IHC.Method: This research has been done with keywords mass spectrometry , MALDI IMS and immunohistochemistry during the January 2015 to July 2019 by analyzing articles from dependable scientific databases including Google Scholar, Science Direct, PubMed, MEDLINE, and Scopus. Result: MALDI MS analysis is applied to intact proteins after separation by isoelectric focusing and alternatively combined with the results of a bottom-up analysis of gel-separated protein digests by MALDI MS. new efforts in improving the sample targets in laser-based desorption/ionization for MS analysis, and a new low-cost disposable metal-coated polymeric sample target plate and a highly porous silver foil as sample substrate for the efficient analysis of lipids. In the field of IF and IHC, exploiting physical characteristics have led to the advent of a series of techniques that have improved spatial resolution by limiting diffraction, such as confocal scanning laser microscopy or super-resolution microscopy techniques. In the very nearby future, variability in human understanding might benefit from digital image quantification performed on scanned slides. Conclusion: There is a great need for multiplex systems that can measure several proteins at once. Oncology might benefit from techniques where predictive biomarkers are quickly analyzed in parallel. DNA markers have made their way into laboratories by PCR and Sanger sequencing. It is time for the proteins to track the course.کلیدواژه ها
MALDI IMS , immunohistochemistry , mass spectrometryمقالات مرتبط جدید
- تولید آنتی بادی پلی کلونال مونواسپسیفیک جهت شناسایی باکتریهای جنس سالمونلا
- بررسی فراوانی سویه های مدرن و اجدادی مایکوباکتریوم توبرکلوزیس بر اساس نشانگر TbD۱ در شمال غرب ایران
- The impact of the gut microbiome on metabolic, psychiatric, and immune diseases
- پیشگیری از سرطان خون سفری به سوی زندگی سالمتر
- Investigating the Effect of Pulegone and Neomenthol Compounds in Cancer Inhibition Using Molecular Docking
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.