Proteome Profiling of Early-Stage Serous Ovarian Cancer

Background: Serous carcinoma, the subtype of ovarian cancer has the highest occurrence and mortality in women. Proteomic profiling using mass spectrometry (MS) has been used to de-tect biomarkers in obtained tissue from patients with ovarian cancer. Thus, this study aimed at analyzing the interactome (protein-protein interaction (PPI)) and (MS) data to inspect PPI networks in patients with early-stage serous ovarian cancer.Materials and Methods: For proteome profiling in early stage serous ovarian cancer, 2DE and mass spectrometry were used. Differentially expressed proteins which had been determined in early-stage serous ovarian cancer and experimental group separately were integrated with PPI data to construct the Query-Query PPI (QQPPI) networks.Results: 6 Hub-bottlenecks proteins with significant centrality values, based on centrality parameters of the network (De-gree and between), were found includingTransgelin (TAGLN), Keratin (KRT14), Single peptide match to actin, cytoplasmic 1(ACTB), apolipoprotein A-I (APOA1), Peroxiredoxin-2 (PRDX2), and Haptoglobin (HP).Conclusion: This study showed these six proteins were intro-duced as hub-bottleneck protein. Itcan be concluded that regu-lation of gene expressioncan have a critical role in the pathol-ogy of early- stage serous ovarian cancer.

کلیدواژه ها:

Proteome Profiling ، Early Stage ، Serous Ovarian Cancer