A Verdi - Department of Reproduction Biology, ACECR, Qom, Iran
S Sahraei - Department of Reproduction Biology, ACECR, Qom, Iran
E ASA - Department of Reproduction Biology, ACECR, Qom, Iran
R Jannatifar - Department of Reproduction Biology, ACECR, Qom, Iran

Background: FSH plays an important and essential role in spermatogenesis, maintaining and integrating the DNA sperm. Several studies showed that infertile men with normal semen parameters have low levels of stress oxidative while infertile men with abnormal semen parameters have more stress oxida-tive level. The aim of this study was to evaluate whether the administration of recombinant FSH (Gonal-f) could improve sperm parameters. The secondary endpoints of this study were to evaluate stress oxidative and Malondialdehyde Levels in oli-gozoospermia infertile men.Materials and Methods: In the present study, an intervention-al study was carried out with a sampling of 50 infertile men (oligozoospermia). The semen samples were examined in ac-cordance to the 2010 World Health Organization criteria. Then before and after treatment with rhFSH (Gonal F) sperm param-eters was determined by light microscopy and oxidative stress were measured with flowcytometry assay. Also Malondialde-hyde Level was evaluated with TBA assay.Results: Measurement of sperm parameters (concentration, motility and morphology) in oligozoospermia patients before and after rhFSH treatment was significantly different and im-proved (P<0.001). Also, the oxidative stress and Malondial-dehyde Levels of seminal plasma significantly decreased after rhFSH treatment (P <0.05).Conclusion: According to the above results, rhFSH treatment has a beneficial effect on sperm parameters in oligozoospermia males and dramatically reduces oxidative stress. Also, increas-ing Malondialdehyde level could be a negative effect of oxi-dative stress on sperm quality and performance. In this study, Malondialdehyde Level also showed a significant decrease after treatment.

Oxidative Stress level, Malondialdehyde level, FSH Treatment, Oligozoospermia