E ASA - Department of Reproductive Biology, ACECR, Qom, Iran
A VERDI - Department of Reproductive Biology, ACECR, Qom, Iran
R Janati far - Department of Reproductive Biology, ACECR, Qom, Iran

Background: To evaluate efficiency of cult active medium on fertilization and cleavage rates, embryo development, and pregnancy rate after intracytoplasmic sperm injection (ICSI) in obstructive azoospermia patients.Materials and Methods: This study was done on 157 ICSI cycles (stimulated with standard long protocol). After oocyte collection, the oocytes were randomly divided into two groups:control and artificial oocyte activation (AOA). The injected oo-cytes in the control group were cultured in cleave. The remain-ing oocytes were chemically activated by exposure to 200 µl cult-active medium for 15 minutes. Around 16 to 18 hours after ICSI, fertilization was assessed. The percentage of cleavage and embryo quality were calculated 72 hours after ICSI. Preg-nancy rate was determined by Biochemical experiment.Results: There are significant differences in the fertilization and cleavage rates after Cult-active used (P<0.05). also the pregnancy rate significantly increased (P<0.05). embryo quality no difference between ICSI AOA and control groups (P> 0.05) Conclusion: The findings showed that Cult-active treatment may fertilization and cleavage rates, which in turn, affect the implantation and pregnancy rate.

Cult-Active, Obstructive Azoospermia, Failed Ferti-lization, Oocyte Activation, ICSI