The Effect of Cult-Active Medium on Fertilization and Pregnancy Rates in Couples with Azoospermia Male Factor
- سال انتشار: 1398
- محل انتشار: بیستمین کنگره بین‌المللی بیولوژی تولید مثل و پانزدهمین کنگره بین‌المللی سلول های بنیادی
- کد COI اختصاصی: RROYAN20_282
- زبان مقاله: انگلیسی
- تعداد مشاهده: 326
نویسندگان
Department of Reproductive Biology, ACECR, Qom, Iran
Department of Reproductive Biology, ACECR, Qom, Iran
Department of Reproductive Biology, ACECR, Qom, Iran
چکیده
Background: To evaluate efficiency of cult active medium on fertilization and cleavage rates, embryo development, and pregnancy rate after intracytoplasmic sperm injection (ICSI) in obstructive azoospermia patients.Materials and Methods: This study was done on 157 ICSI cycles (stimulated with standard long protocol). After oocyte collection, the oocytes were randomly divided into two groups:control and artificial oocyte activation (AOA). The injected oo-cytes in the control group were cultured in cleave. The remain-ing oocytes were chemically activated by exposure to 200 µl cult-active medium for 15 minutes. Around 16 to 18 hours after ICSI, fertilization was assessed. The percentage of cleavage and embryo quality were calculated 72 hours after ICSI. Preg-nancy rate was determined by Biochemical experiment.Results: There are significant differences in the fertilization and cleavage rates after Cult-active used (P< 0.05). also the pregnancy rate significantly increased (P< 0.05). embryo quality no difference between ICSI AOA and control groups (P> 0.05) Conclusion: The findings showed that Cult-active treatment may fertilization and cleavage rates, which in turn, affect the implantation and pregnancy rate.کلیدواژه ها
Cult-Active, Obstructive Azoospermia, Failed Ferti-lization, Oocyte Activation, ICSIاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.