Different Gene Expression of Lipopolysaccharide Induce Tumor Necrosis Factor-Alpha Factor (LITAF) in Endometrial Tissues of Women with and without Endome-triosis

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 323

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شناسه ملی سند علمی:

RROYAN20_246

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Endometriosis is an estrogen-dependent and chronic inflammatory disease of women in reproductive age. Endometriosis is defined as the presence of endometrial glands and stroma outside the uterine cavity. Immune system altera-tions in peritoneal cavity is one of the main due of growth and proliferation of endometrial lesions in women with endome-triosis. It is shown that macrophages number and activation are increased in peritoneal fluid of women with endometriosis. Tumor necrosis factor-α (TNF-α) is one of the main inflamma-tory cytokines secreted by macrophages. It has been reported that the concentration of TNF-α is increased in peritoneal fluid of women with endometriosis. Our study showed that gene expression of TNF-α is increased in ectopic lesions of women with endometriosis in compare to control group. Lipopolysac-charide induce tumor necrosis factor-alpha factor (LITAF) is a transcription factor that regulates the expression of TNF-α gene. The objective of present study was gene expression of LITAF in eutopic and ectopic endometrial tissues of women with endo-metriosis in compare to women without endometriosis.Materials and Methods: In this study, ten women with endo-metriosis (endometriosis group) and 10 women without endo-metriosis (control group) were enrolled after signing consent form. Both groups underwent laparoscopy surgery at Royan Institute. Ectopic endometrial samples were collected during laparoscopy. Eutopic and control endometrial samples were collected by pipelle. Then, RNA extraction and cDNA synthe-sis were done for all the tissues. Gene expression of LITAF was measured by real time-PCR. P value less than 0.05 was consid-ered statistically significant.Results: Ectopic endometrial tissues showed highest LITAF gene expression level in compare to eutopic and control sam-ples. LITAF gene expression was significantly higher in ectopic tissues in compare to control samples. In addition, the level of LITAF gene expression was higher in eutopic endometrial tis-sues of women with endometriosis than control group but this difference was not significant.Conclusion: The results of this study suggest that over expres-sion of LITAF in endometrial tissues of women with endometri-osis could involve in inflammatory pathways of endometriosis.

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نویسندگان

A Saadat Varnosfaderani

Department of Cell and Molecular Biology Science, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reprod

SH Kalantari

Department of Cell and Molecular Biology Science, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reprod

F Ramezanali

Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

M Shahhoseini

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran