The Effect of Methanolic Extract of Poulak on Mean Volume and Total Number of Leydig Cells and Plasma Level of Testosterone in Type 2 Diabetic Rats
- سال انتشار: 1398
- محل انتشار: بیستمین کنگره بین‌المللی بیولوژی تولید مثل و پانزدهمین کنگره بین‌المللی سلول های بنیادی
- کد COI اختصاصی: RROYAN20_233
- زبان مقاله: انگلیسی
- تعداد مشاهده: 326
نویسندگان
Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده
Background: Diabetes mellitus is a common metabolic disor-der of endocrine system which increasing so dramatically all over the world and can gradually cause damage to male re-productive system. Stachys schtschegleevii which commonly known as poulak has been used in Iranian traditional medicine due to its analgesic, antibacterial, anti-inflammatory, antioxi-dant and hypoglycemic properties. The aim of this study was to evaluate the effect of methanolic extract of poulak (MEP) on the number, morphology and function of Leydig cells in dia-betic male rats.Materials and Methods: Thirty adult male rats were divided into 6 groups including: control, diabetic, diabetic treated by glibenclamide and diabetic treated by 100, 200 and 300 mg/kg MEP for 40 consecutive days by oral gavage. Type II diabetes was induced by high fat diet and low dose of streptozotocin (STZ). After 2 weeks of dietary manipulation, and an overnight fasting, the rats were injected intraperitoneally with 35 mg/kg STZ. At the end of experiment, animals were anaesthetized and plasma samples were taken from caudal vena cava for measure-ment of testosterone and then testes were removed and fixed in 10% neutral buffered formalin. Tissue were processed by standard paraffin embedding and serially sectioned at 20 µm thickness. Selected sections by systematic random sampling were stained with H&E and mean volume and total numbers of Leydig cells were estimated by optical dissector and stereo-investigator software. Finally, one-way ANOVA and Tukey s post-hoc were performed for data analysis.Results: Results showed that diabetes reduced mean volume and total number of Leydig cells as well as plasma level of testosterone significantly compared to control groups. It was also indicated that administration of MEP could improve and increase size and number of Leydig cells and testosterone level of diabetic animals in a dose dependent manner. Our analysis revealed that all doses of poulak have better effect than gliben-clamide treated groups.Conclusion: Based on our results it can be concluded that ad-ministration of poulak due to improvement of structure and function of Leydig cells and enhancement of spermatogenesis process can be considered as a suitable protective strategy for increasing of fertility in diabetic males.کلیدواژه ها
Poulak, Stachys Schtschegleevii, Diabetes, Leydig Cells, Testosteroneمقالات مرتبط جدید
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