Supplementation of Maturation Medium with CoQ10 Enhances Developmental Competence of Ovine Oocytes Through Improvement of Mitochondrial Function
- سال انتشار: 1398
- محل انتشار: بیستمین کنگره بین‌المللی بیولوژی تولید مثل و پانزدهمین کنگره بین‌المللی سلول های بنیادی
- کد COI اختصاصی: RROYAN20_220
- زبان مقاله: انگلیسی
- تعداد مشاهده: 342
نویسندگان
Reproductive Biotechnology Research Center, Avicenna Re-search Institute, ACECR, Tehran, Iran
Department of Reproductive Biotechnology, Reproductive Bio-medicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Reproductive Biotechnology, Reproductive Bio-medicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Department of Reproductive Biotechnology, Reproductive Bio-medicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
چکیده
Background: In vitro maturation (IVM) can impair the balance between anti-oxidant capacity and oxidative stress, which may jeopardize embryo development by increasing oxidative stress, reducing energy metabolism and causing improper meiotic seg-regation. Balance in energy production and reduction of oxida-tive stress can be achieved by supplementation of Coenzyme Q10 (CoQ10), an electron transporter in the mitochondrial in-ner membrane. With the aim to improve the in vitro production of ovine embryos, we studied the effect of CoQ10 supplementa-tion during the maturation sheep oocytes.Materials and Methods: A minimum of 100 cumulus oocyte complexes (COCs) were matured in the presence of 15, 30 or 50 μM CoQ10 in three to five replicates, followed by in vitro fertilization and culture in a subset of oocytes.Results: Our data revealed that when compared to control oo-cytes or other concentrations of CoQ10, supplementation with 30 µM CoQ10 resulted in a significant increase in blastocyst and hatching rates, improved the distribution, relative mass and potential membrane of mitochondria, decreased levels of reac-tive oxygen species (ROS) and glutathione (GSH) and also the percentage of oocytes with misaligned chromosomes after spin-dle assembly. The relative expression levels of apoptosis mark-ers CASPASE3 and BAX were significantly reduced in treated oocytes and cumulus cells while the relative expression level of GDF9, an oocyte specific growth factor, significantly increased in treated cumulus cells and oocytes.Conclusion: In conclusion, supplementation with CoQ10 im-proves the quality of COCs and the subsequent developmental competence of the embryo.کلیدواژه ها
Coenzyme Q10, In Vitro Maturation, Mitochondrial Status, Oocytes, Oxidative Stressاطلاعات بیشتر در مورد COI
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