The Effect of Extracellular Vesicles Derived from Chondrocyte and Mesenchymal Stem Cells on Chondrogen-esis; An In Vitro Study
- سال انتشار: 1398
- محل انتشار: بیستمین کنگره بین‌المللی بیولوژی تولید مثل و پانزدهمین کنگره بین‌المللی سلول های بنیادی
- کد COI اختصاصی: RROYAN20_078
- زبان مقاله: انگلیسی
- تعداد مشاهده: 266
نویسندگان
Department of Stem Cells and Developmental Biology, Royan In stitute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Stem Cells and Developmental Biology, Royan In stitute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Stem Cells and Developmental Biology, Royan In stitute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
چکیده
Background: Osteoarthritis (OA) is the most prevalent joint disease worldwide. Given the absence of vasculature and pro-genitor cells, cartilage is unable to self-repair. Recently, ex-tracellular vesicles (EV) have been suggested as a promising therapeutic approach to treat OA. However, it is necessary to identify an appropriate cellular source for isolation of extracel-lular vesicles with chondrogenic ability in order to promote car-tilage regeneration.Materials and Methods: Hence, this study is aimed to evaluate chondrogenic potential of EV derived from different cellular sources under in vitro condition. We isolated EV from rabbit chondrocyte (Cho), and bone marrow mesenchymal stem cells (rBMSCs) using a consistent centrifugation protocol. Isolat-ed EVs from all groups were characterized in terms of size, morphology and surface markers by dynamic light scattering (DLS), scanning electron microscope (SEM) and western blot-ting, respectively. The isolated EV were added into micromass-es composed of rBMSCs and allowed to differentiate for 21 days. Quantitative real time-PCR (qRT-PCR) and histologicalanalysis were subsequently performed to assess the quality of chondrogenic differentiation among experimental groups. Results: The size of isolated EVs from Cho, and rBMSCs was 151.4 ± 498.4, and 163.3 ± 1.7 nm, respectively. Western blot analysis confirmed the expression of specific markers including CD9 and CD81 in isolated EVs while annexin was not detected. The qRT-PCR results showed up-regulated expression level of Col II as a major chondrogenic marker in a group treated by rBMSCs compared to control and Cho groups. In contrary, the expression level of ColX decreased in both group. Safranin O and toluidine blue staining were positive for the group, which received rBMSCs –EVs, confirming deposition of proteogly-can.Conclusion: Based on the results of the current study, we con-cluded that EVs isolated from MSCs would efficiently improve chondrogenic differentiation of rBMSCs and accelerate carti-lage regeneration.کلیدواژه ها
Extracellular Vesicles, Chondrogenesis, Bone Mar-row Mesenchymal Stem Cell, Chondrocyteاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.