The protective effect of vitamin C on phenylhydrazine-induced hemolytic anemia on sperm quality and in-vitro embryo development in mice
- سال انتشار: 1397
- محل انتشار: International Journal of Reproductive BioMedicine، دوره: 16، شماره: 12
- کد COI اختصاصی: JR_IJRM-16-12_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 372
نویسندگان
Ph.D., Department of Anatomy and Histology, Faculty of Medicine, Urmia University of Medical Sciences,Urmia, Iran
M.Sc., Department of Anatomy and Histology, Faculty of Medicine, Urmia University of Medical Sciences,Urmia, Iran
Ph.D., Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Ph.D., Department of Anatomy and Histology, Faculty of Medicine, Urmia University of Medical Sciences,Urmia, Iran
چکیده
Background: Phenylhydrazine (PHZ) induced anemia and was shown to have harmfuleffects on the male reproductive system.Objective: To investigate the protective effect of vitamin C (Vit C) on sperm parametersquality, in vitro fertilization potential and embryonic development in a mouse model ofhemolytic anemia induced by PHZ.Materials and Methods: Thirty-two NMRI adult male mice (n = 8/each) were randomlyclassified into four groups. Group I (control) received normal saline, Group II (PHZ)received 8 mg/100 gr body weight PHZ as initial dose, continued by 6 mg/100gr intraperitoneally every 48 hr, Group III (Vit C) received Vit C (10 mg/kg, daily,intraperitoneally), and group IV (PHZ + Vit C) received PHZ and Vit C. After 35 days,sperm quality parameters, the percentage of sperm with DNA damage and in vitrofertilization outcomes up to blastocyst stage were evaluated.Results: A significant (p < 0.001) reduction in all of the sperm parameters (count,motility, viability and normal morphology) were observed in group II (PHZ) comparedwith group I (control). In group IV (PHZ ± Vit C), these parameters and sperm DNAdamage (p < 0.001) improved significantly when compared with PHZ-treated mice.Furthermore, PHZ caused a significant (p < 0.001) decrease in the fertilization rateand the percentage of pre-implantation embryos’ (two cell embryo and blastocyst)formation in comparison to group I (control), and Vit C supplementation in mice of groupIV improved significantly the fertilization rate (p = 0.002), but it could not improve thepercentage of two cell embryos and blastocyst production.Conclusion: The data from this study indicated that Vit C decreased the adverseeffects of PHZ on the quality of sperm parameters and in vitro fertilization rate, butit is insufficient to restore the in-vitro embryonic development and fertility potential.کلیدواژه ها
Vitamin C, Phenylhydrazine, Sperm, Fertilization, Miceاطلاعات بیشتر در مورد COI
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