Kidney therapeutic potential of peptides derived from the bromelain hydrolysis of green peas protein
- سال انتشار: 1398
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 22، شماره: 9
- کد COI اختصاصی: JR_IJBMS-22-9_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 356
نویسندگان
Faculty of Medicine, Universitas Kristen Maranatha, Bandung Indonesia
Faculty of Medicine, Universitas Kristen Maranatha, Bandung Indonesia
Faculty of Medicine, Universitas Kristen Maranatha, Bandung Indonesia
School of Pharmacy, Institute Teknologi Bandung Indonesia
چکیده
Objective(s): Kidney disease is a global health problem that needs a solution to its therapy. In the previous study, we found that protein hydrolysate of green peas origin of Indonesia hydrolysed by bromelain (PHGPB) showed improve kidney function in cisplatin-induced nephropathy rats. In this study, we investigated the effect of PHGPB to obtain effective dose that exerts a therapeutic effect on chronic kidney disease (CKD) based on reducing urea and creatinine levels and to elucidate its mechanism of action. Materials and Methods: Two sets of experiments were conducted: (1) characteristics and proteomic profile of PHGPB, (2) in vivo test of PHGPB in gentamycin-induced Wistar rats, including urea and creatinine measurements, activities of antioxidant and kidney-related peptides (ANP, COX-1, and renin).Results: PHGPB showed three bands under 10 kDa using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and contained 10 identified proteins using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Significant differences in urea and creatinine levels were found between all PHGPB treatments and positive controls (P< 0.01). The lowest levels of urea and creatinine that were validated by high super oxide dismutase (SOD) activity and atrial natriuretic peptide (ANP) level were obtained in the 200 mg/day PHGPB treatment. However, the mean renin level was high and cyclooxygenase-1 (COX-1) level did not exceed positive and negative control levels. Conclusion: PHGPB at dose 200 mg/kgBW shows a potential CKD therapeutic effect that is dose-dependent. Higher PHGPB dose corresponds to better effect on kidney function by increasing antioxidant activity and ANP levels in gentamycin-induced Wistar rats.کلیدواژه ها
ANP, Bromelain, COX-1, Protein hydrolysate green pea, Renal function, Renin, SODاطلاعات بیشتر در مورد COI
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