The effect of Origanum vulgare extract on kinetics parameters of cryopreserved ram sperm

The aim of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on kinetics parameters of post-thawed Moghani ram sperm. Four Moghani ram were used for semen collection twice a week by an artificial vagina and ejaculates with same condition were pooled. Different levels of ethanol extract of Thymus vulgaris (0, 2, 4, 8, 12 and 16ml in dL diluents solution) were added to Tris based diluents. Following cooling and freezing of semen samples, they were stored in liquid nitrogen until evaluation. After freezing-thawed, the dynamic parameters were evaluated using CASA system. results showed that 2 and 4 mL/dL Thymus vulgaris extract had higher percent total motility (57.92 ±4.56% and 55.38 ±4.17%) compared to other groups (P < 0.05). Extended semen supplemented with 4 mL/dL extract exhibited higher (P < 0.05) percentages of progressive motility compared to control, 12 and 16 mL/dL extract groups. The percentage of VSL was higher (P<0.05) in the extender containing 4 and 8 mL/dL extract compared to the other groups. 4 mL/dL extract caused significant increase in VCL parameter in comparison to the control, 12 and 16 mL/dL extract groups (P < 0.05). VAP parameter level was higher (p < 0.05) in 4 mL/dL compared to 16 mL/dL extract. For parameters of progressive motility and VSL, LIN and STR the lowest performance (P<0.05) was observed at 16 mL/dL of extract. In conclusion, supplementation of extender with 4 mL/dL Thymus vulgaris L. extract improves kinetics parameters of post-thawed ram sperm.

کلیدواژه ها:

Antioxidant ، Thymus vulgaris ، Semen ، Freezing-thawed