Evaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes

  • سال انتشار: 1393
  • محل انتشار: مجله گیاهان دارویی ابن سینا، دوره: 4، شماره: 5
  • کد COI اختصاصی: JR_AJP-4-5_005
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 529
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نویسندگان

Kobra Shirani

Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

Javad Behravan

Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

Fatemeh Mosaffa

Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

Mehrdad Iranshahi

Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, I. R. Iran

چکیده

Objective: Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F.szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes. Materials and Methods: Human lymphocytes were incubated with H2O2 (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H2O2 (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H2O2 were used as negative controls. Results: The findings of this study demonstrated that H2O2 exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H2O2-induced oxidative DNA damage in human lymphocytes. Conclusion: According to the results, it is concluded that the capability of F. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.

کلیدواژه ها

Comet assay, DNA damage, Galbanic acid, Ferula szowitsiana

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