Antifungal, antibacterial, antioxidant, and cytotoxic effects of Fumaria officinalis aqueous extract

Fumaria officinalis has been used in traditional medicine as a therapeutical supplement. The purpose of the recent research was to assess the antifungal (against Candida albicans, Candida glabrata, Candida parapsilosis, Candida krusei, Candida guilliermondii), antibacterial (against Escherichia coli O157:H7, Pseudomonas aeruginosa, Klebsiella pneumonia, Listeria monocytogenes, Shigella flexneri, Proteus mirabilis, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus pyogenes, Bacillus subtilis, and Enterococcus faecalis), antioxidant, and cytotoxic effects of F. officinalis aqueous extract. Materials and Methods: After identification of the plant, its aqueous extract was obtained using Soxhlet extractor without leaving any chemicals in it. Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. In agar disk diffusion test, distilled water was used as negative control, while antifungal (Fluconazole, Itraconazole, Miconazole, Amphotericin B, and Nystatin) and antibacterial (Difloxacin, Chloramphenicol, Streptomycin, Gentamicin, Oxytetracycline, Ampicillin, and Amikacin) antibiotics were used as positive controls. Findings: Indeed, compared with all standard antibiotics, the F. officinalis aqueous extract showed higher antifungal and antibacterial properties (p<0.01). DPPH free radical scavenging test was carried out to examine the antioxidant property, which revealed similar antioxidant property of F. officinalis with butylated hydroxy toluene (BHT) as a positive control. The synthesized aqueous extract had great cell viability dose-dependently (Investigating the effect of the plant on Hela, MCF-7, and HEK-293 cell lines) and indicated this method was nontoxic for synthesizing F. officinalis. Conclusion: Seemingly, because of aqueous extract of F. officinalis antifungal, antibacterial, antioxidant, and non-cytotoxic properties, it can be used as a medical supplement

کلیدواژه ها:

Fumaria officinalis ، aqueous extract ، antifungal ، antibacterial ، antioxidant ، cytotoxicity