The Effect of Vitamin A on MCF-7 Cell Viability

Introduction and Aim: Vitamin A is a fat soluble vitamin and a potent antioxidant that helps facilitate cellular differentiation and strengthens the immune system. Beta-carotene is a potent antioxidant that enhances the immune system and prevents many chronic diseases. The use of vitamin A prevents the development of cancer cells and, as a result, helps fight cancer. Retinoic acid plays an important role in the growth and differentiation of cells and can be a weapon against cancer. The aim of this study was to evaluate the effect of vitamin A in different concentrations on apoptosis in the MCF-7 cell line. Methods: MCF-7 cells were seeded and treated with 0.01, 0.1, 1 mg/ml concentrations of vitamin A for 1, 3 and 5 days. Cell viability was evaluated on certain days after treatment. We used MTT assay to confirm cell viability. The absorbance of plates was measured at 570 nm using an ELISA reader. Results: Our observations didn t show a significant change in cell viability of treated cells with vitamin A. Conclusion: Therefore, we conclude that vitamin A doesn t have anticancer effect on MCF-7 cells and needs further research.

کلیدواژه ها:

Vitamin A ، MCF-7 ، Cell Viability