An investigation of the effect of the human cumulus cell conditioned medium on mouse oocytes in vitro maturation

Background: Since defect of oogenesis is the cause of infertility in some infertile couples, in vitro maturation (IVM) of germinal vesicle (GV) oocytes is an important option in infertility treatments. Cumulus cells are essential for nutrition of oocytes during oogenesis. They provide a suitable environment contains growth factors required for oogenesis. It was shown that cumulus cell conditioned medium (CCCM) supports female germ cell development from Buffalo embryonic stem cells (ESCs).Objective: In this study the impact of CCCM on murine IVM was investigated.Materials and methods: NMRI female mice, aged 4-6 weeks, were injected with 5 IU of Pregnant Mare’s Serum Gonadotropin (PMSG) intrapritoneally (IP). 240 germinal vesicle (GV) oocytes were obtained. GV oocytes without cumulus cells were cultured in CCCM (test group; 120 GVs) and DMEM+10%FBS (control group; 120 GVs). GV maturation rate was checked by microscopic observation at 8, 16 and 24 hours following IVM in both groups.Results: Significant difference was observed between CCCM (19.16%) and control (0%; p<0.05) following IVM.Conclusion: Human CCCM supports IVM in mice oocytes. For further investigations we aim to evaluate the effect of TCCM on human IVM and assess the metabolomics profile of TCCM in our future studies.

کلیدواژه ها:

Conditioned Medium ، Cumulus Cells ، Germinal Vesicle ، In Vitro Maturation