Is there any correlation between embryo morphokinetics development and maternal age in an intra-cytoplasmic sperm injection program

Background: It is widely accepted that aging decreases the fertility capacity in women.Objective: The aim of this study was to assess embryo morphokinetics parameters and cleavage pattern correlation with maternal age.Materials and Methods: Morphokinetics of embryos derived from women <30, 30-35, 35-40 and 40≤yr were compared retrospectively in terms of time of second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf) and time of two to eight discrete cells (t2-t8). Also, abnormal cleavage patterns such as, uneven blastomeres at two cells stage, cell fusion (Fu) and Trichotomous Mitoses (TM) were assessed.Results: The findings showed that only t5 occurred later in women 35-40 and 40≤ when compared with <30 and 30-35 yr (p=0.000). Other morphokinetics timing parameters as well as uneven blastomere were comparable (p> 0.05). However, Fu and TM were increased in women with 40≤ age compared with younger women (p=0.000).Conclusion: It is concluded that maternal age correlated to cleavage pattern of embryos. So, evaluation of embryo morphokinetics may improve embryo selection and increase fertility in advanced maternal age.

کلیدواژه ها:

Morphokinetics ، Cleavage pattern ، Maternal age ، Embryo