Wnt signaling pathway in uterus of normal and seminal vesicle excised mated mice during pre-implantation window
- سال انتشار: 1398
- محل انتشار: هشتمین کنگره بین المللی و جشنواره دانشجویی طب تولید مثل و سومین کنگره بین المللی ژنتیک تولید مثل
- کد COI اختصاصی: RMED08_121
- زبان مقاله: انگلیسی
- تعداد مشاهده: 460
نویسندگان
Department of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Reproductive Biology, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Background: The importance of seminal vesicle secretion and uterine Wnt signaling for uterus preparation and embryo implantation has been described.Objective: The aim of this study was evaluating effect of seminal vesicle secretion on uterine Wnt signaling.Materials and Methods: In this study, we evaluated the gene expression of Wnt ligands (Wnt4 and Wnt5a) and their corresponding receptors (Fzd2 and Fzd6) using qRT-PCR and active beta-catenin protein levels using western blotting in the uterine tissue of female mice mated with intact and seminal vesicle-excised (SVX) males during the pre-implantation window. We evaluated the association between these factors and implantation rates and embryo spacing.Results: mRNA expression of Wnt4 and Wnt5a and active beta-catenin protein levels decreased from Day 1 to Day 4, but reached a peak on the fifth day of pregnancy. Fzd2 also reached its highest level on Day 5. Fzd6 expression showed a decreasing trend towards the day of implantation. Lack of seminal vesicle secretion decreased Wnt4 and Wnt5a expression on Days 1 and 5 and beta-catenin levels on Day 5. There were almost no significant differences in expression levels of the Fzd2 and Fzd6 receptors between groups. There were positive and negative correlations, respectively, between implantation rates and embryo spacing and Wnt4, Wnt5a and active beta-catenin in the control group, but such correlations were not observed in the SVX-mated mice.Conclusion: Significant changes occurred in the expression of several Wnt signaling members and there was a significant association between Wnt signaling and embryo implantation. Seminal vesicle secretion affects Wnt signaling in mice and consequently also affects murine embryo implantation.کلیدواژه ها
Wnt Signaling, Seminal vesicle, Embryo implantationاطلاعات بیشتر در مورد COI
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