Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide
- سال انتشار: 1398
- محل انتشار: هشتمین کنگره بین المللی و جشنواره دانشجویی طب تولید مثل و سومین کنگره بین المللی ژنتیک تولید مثل
- کد COI اختصاصی: RMED08_098
- زبان مقاله: انگلیسی
- تعداد مشاهده: 459
نویسندگان
Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
چکیده
Background: Reproductive toxicity is one of the side effects of cyclophosphamide (CP) in cancer treatment. Pumpkin seeds and Zingiber officinale are natural sources of antioxidants.Objective: We investigated the possible protective effect of combined pumpkin seed and Zingiber officinale extracts on sperm characteristics, epididymal histology and biochemical parameters of CP-treated rats.Materials and Methods: Male adult Wistar rats were divided randomly into six groups. Group 1, as a control, received an isotonic saline solution injection intraperitoneally (IP). Group 2 were injected IP with a single dose of CP (100 mg/kg) once. Groups 3 and 4 received CP plus 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract (50:50). Groups 5 and 6 received only 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract. Six weeks after treatment, sperm characteristics, histopathological changes and biochemical parameters were assessed.Results: In CP-treated rats, motile spermatozoa were decreased, and abnormal or dead spermatozoa increased significantly (p< 0.001) but administration of the mixed extract improved sperm parameters. Epididymal epithelium and fibromascular thickness were also improved in extract-treated rats compared to control or CP groups. Biochemical analysis showed that the administration of combined extracts could increase the total antioxidant capacity (TAC) level significantly in groups 3, 4, 5 and 6. Interestingly, the mixed extract could decrease most of the side effects of CP such as vacuolization and separation of epididymal tissue.Conclusion: Our findings indicated that the combined extracts might be used as a protective agent against CP-induced reproductive toxicity.کلیدواژه ها
Cyclophosphamide, Pumpkin seed and Zingiber officinale extract, Rat’s epididymis, Sperm parameters, Total antioxidant capacityاطلاعات بیشتر در مورد COI
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