Carob promotes spermatogenesis in infertile mice model via inducing the self-renewal process in spermatogonial stem cells

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 509

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

این مقاله در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

RMED08_061

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: Herbal medicine, also called botanical medicine or phytomedicine, refers to using a plant’s seeds, leaves and others for medicinal purposes. Herbs are widely used today, in teas, vitamins and natural supplements. While the benefits of herbal medicine are vast, it is important to understand the basis of herbal medicine. Ceratonia silique l.(Carob) extract is being traditionally used for male infertility treatments. However, there is not sufficient scientific basis for using this herb and the mechanism underlying its function yet to be understand.Objective: Herein, we evaluated the Carob extract in infertile mice model and uncovered the mechanism underlying its function.Materials and Methods: In this study, 160 male mice (mean age: 6-8 wk) were divided into five groups as follow: intact, vehicle, positive control and four experiment groups that received different doses of Carob extract by oral gavage for 35 days. For creation sterile model, we injected a single dose of 45 mg/kg bw busulfan into the peritoneum. The positive control was administered 25 mg/kg bw of Clomiphene citrate and Carob groups received oral gavage doses: 75, 150, 300 and 600 mg/kg bw. The sperm parameters, testicular histopathology, DNA content, ROS level as well as gene expression analysis for spermatogenesis-related genes were investigated after 35 days of the extract.Results: Our results demonstrated that Carob administration significantly increased sperm count and motility in a dose dependent manner. Moreover, this extract significantly reduced the DNA fragmentation and ROS level in sperm cell population. Histological analysis revealed increased spermatogenesis following carob extract gavage. Gene expression analysis confirmed significant increase in the expression levels of spermatogonial stem cells (SSCs) self-renewal genes that including Plzf, Gfr-1, Bcl-6b, Utf-1 as well as differentiating-related genes that consist of Dazl, C-kit, Ngn3, Stra8.Conclusion: We have shown that, carob as a natural compound have the ability to induce spermatogenesis in infertile mice model. Our results suggest that Carob promotes both self-renewal of SSCs and differentiation of spermatogonia that are necessary to support the spermatogenesis. Our primary data suggest the Carob as an effective herbal medicine that can be used for non-obstructive azoospermia men. However, more investigation needs to demystify its function before applying it in clinic.

کلیدواژه ها:

Cardiomyocyte ، Differentiation ، Human embryonic stem cells ، Toll like receptors

نویسندگان

Z Ghorbaninejad

Department of Developmental Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Ste

A Eghbali

Department of Developmental Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Ste

P Eftekhari Yazdi

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

F Esfandiari

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran