In vitro analysis of the anticancer properties of cobra venom in colorectal cancer cell line
- سال انتشار: 1397
- محل انتشار: چهارمین کنگره بین المللی سرطان های دستگاه گوارش
- کد COI اختصاصی: CANCERMED04_058
- زبان مقاله: انگلیسی
- تعداد مشاهده: 665
نویسندگان
Biotechnology Research Center, Medical Biotechnology Department, Venom and Biotherapeutics Molecules Lab., Pasteur Institute of Iran. Tehran-Iran.
Biotechnology Research Center, Medical Biotechnology Department, Venom and Biotherapeutics Molecules Lab., Pasteur Institute of Iran. Tehran-Iran.
Biotechnology Research Center, Medical Biotechnology Department, Venom and Biotherapeutics Molecules Lab., Pasteur Institute of Iran. Tehran-Iran.
چکیده
Introduction & Aim: Cancer is the most important cause of death in the world. Despite the many therapeutic approaches used to treat cancer, chemotherapy is still the main method of treating cancer. One of the main problems with chemotherapy is that cancer cells usually resist these drugs and the efficacy of chemotherapeutic drugs decreases. Research on snake venom had led to the discovery of some proteins and compounds for treatment of cancers and other human diseases. Methods: Cobra venom was fractionated by using size exclusion chromatography. The toxic fractions were identified by MTT assay and the effect of this toxic fraction was evaluated on both cancer cells and normal cells. Cell proliferation test was performed in order to evaluate its toxic effect on cancer cells.Results: In size exclusion chromatography of Cobra venom, 7 fractions were obtained from which the fraction 5 contained the most toxic effect on colon cancer cells. This fraction leads to 90% death in colon cancer cells, and suppressed the cell proliferation in the cells whereas it only had small cytotoxicity on normal cells.conclusion: In this study, Iranian cobra venom was found to have cytotoxic effects on tumor cells of the colon and also had very limited effect on normal cells. Separation of cobra venom and investigating the effect of each component of venom can help researchers to find a best compound that can cure colon cancer.کلیدواژه ها
Venom, Cobra, snake, colorectal cancer.مقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.