DNA methylation regulated microRNA-200b in colorectal polyp and cancer tissues in Iranian population
- سال انتشار: 1397
- محل انتشار: چهارمین کنگره بین المللی سرطان های دستگاه گوارش
- کد COI اختصاصی: CANCERMED04_035
- زبان مقاله: انگلیسی
- تعداد مشاهده: 626
نویسندگان
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده
Introduction: In recent years, colorectal cancer (CRC) incidence has been increasing to become a serious reason of mortality worldwide from cancers, with high rates in westernized societies and increasing rates in developing countries. Epigenetic modifications including changes in DNA methylation, histone modifications, and non-coding RNAs play a critical role in carcinogenesis. Aberrant DNA methylation is a common molecular feature in colorectal cancer (CRC). Hypermethylation of miR-200b promoter, as an epigenetic factor, involves in CRC. Methylation of miR-200b has been investigated in CRC and normal tissues but these study in colorectal polyps has evaluated fo the first time. This study investigated miR-200b methylation in colorectal adenomatous polyp, hyperplastic polyp and adenocarcinoma tissues. Materials and Methods In the recent study, miR-200b methylation was examined in 131 samples from 114 individuals, including 30 adenocarcinoma specimens, 17 tumor adjacent normal tissues, 78 primary lesions (55 adenomatous polyps and 23 hyperplastic polyps) and 6 healthy individuals. The methylation status of miR-200b promoter was considered by methylation-specific PCR. Results Methylation of miR-200b was observed in adenocarcinoma samples (86%) and adenomatous polyps (85%), while most of hyperplastic polyps were unmethylated (69.6%). Neither control individuals nor tumor adjacent normal tissues exhibited methylation in miR-200b promoter. Aberrant methylation of miR-200b was significantly more common in tumor tissues and adenomatous polyps than in hyperplastic polyps (p < 0.0001) and tumor adjacent normal tissues (p < 0.0001). Conclusion Methylation status of miR-200b promoter was changed during CRC development and it may be identified as an attractive biomarker for early detection of the disease.کلیدواژه ها
miR-200b, colorectal cancer, polyp, epigenetic, methylation, biomarkerمقالات مرتبط جدید
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