Radial flow bioreactors for expansion of cells: emphasis on kinetic models

Introduction The culture strategy for anchorage-dependent cells is classified into four categories in terms of medium flow and scaffold. The bioreactor design is mainly required for culture of cells attached on scaffold under the medium flow conditions. The environment surroundings of cells consist of three phases; gas (O2, CO2), liquid (medium) and solid (cells, tissues, scaffolds). Objectives It has been reported that SCs have a potential role in the treatment of a wide range of conditions including acute myocardial infarction, graft versus host disease and diabetes, and are currently under investigation in over 300 clinical trials. SCs can be utilized for both autologous therapy (i.e., patient-specific cells) as well as allogeneic therapy (i.e., cells from a universal donor). Since SCs are rare in adult tissues, the harvested cells must be expanded in vitro to generate sufficient numbers for clinical administration. There are several systems to achieve a high cell density in vitro. Results and Conclusion A radial flow bioreactor for cultivating mammalian cells was developed to produce biological materials. The radial flow bioreactor (RFB) is one type of perfusion bioreactor. This type has the ability to maintain an ideal cell culture environment by radial provision of the medium, enabling the construction of comparatively larger tissues. Furthermore, the RFB offers highly functional 3D cultivation. To allow an even distribution of oxygen, the medium is pumped to the center of the chamber from the periphery under low shear stress. Model predictions were presented and analyzed to study the complex dynamics of large cell populations and to determine how the observed proliferation rates are affected by the initial spatial cell distribution, the seeding density, and the characteristics of growth surface.

کلیدواژه ها:

Radial flow bioreactor ، Perfusion bioreactor ، 3D cultivation ، Shear stress