Strontium-based bioactive glasscomposite for bone regeneration

The role of bioactive glass (BG) and glass-ceramics as suitable biomaterials for orthopedic and dental applications as well as in bone tissue engineering (BTE) has been well documented. Strontium (Sr) based compounds can be accumulated in the bone by calcium exchange, playing an important role for the treatment and prevention of osteoporosis and vertebral fractures. Sr is known to accelerate bone tissue repair as well having antibacterial activity.Here, we have investigated the in vitro and in vivo effects of the presence of Sr in the polymer matrix on proliferation and differentiation of bone marrow mesenchymal stem cells with possible application for BTE. The potential angiogenic effect of Sr was also evaluated by subcutaneous implantation in mice. It was hypothesized that Sr incorporation can enhance the stimulating effects of bioactive glass on osteogenesis, angiogenesis and antibacterial activities.Scanning Electron Microscopy of the prepared scaffolds confirmed an interconnected porous structure with an average diameter of 100–300 μm. The stiffness of scaffolds containing Sr was about five times greater than that of samples containing BG with no Sr. Alkaline phosphatase secretion was significantly enhanced in cells cultured on Sr-containing scaffolds, compared with other samples, after 14 days. Imunohistofluorescence analysis indicated that the addition of Sr increased perivascular localization and cell migration, and stimulated sprouting angiogenesis. Our results confirmed that the presence of BG particles could improve mechanical, biological, and angiogenic properties of the polymer matrix which is a promising strategy for creating materials for bone regeneration.

کلیدواژه ها:

Tissue engineering ، Bioactive glass ، Bone graft ، Strontium ، Vascularization