Investigation of the effect of fluid shear stress and low intensity pulsed ultrasound treatment on cancerous bone cells that seeded into 3D βTCP/PLGA porous scaffold: synchronized and separated effects

Introduction Physical factors enhance bone cell growth through mechanotransduction process. Low intensity pulsed ultrasound (LIPUS) and fluid shear stress are mechanical signals that can stimulate bone cells. Objectives The aim of this study was to investigate the synchronized and separated effect of LIPUS and fluid shear stress on cancerous bone model cell inside a β-tricalciumphosphate (β-TCP)/poly lactic-co-glycolic acid (PLGA) 3D-porous scaffold. Methods G-292 osteosarcoma cells were cultured inside the 3D-porous scaffold within the static and perfusion bioreactors with or without LIPUS for 3 or 7 days. The perfusion bioreactor was coupled to the Exogen 4000+ (Smith & Nephew, Inc., London, UK) device probe at the 5mm distance between probe and scaffold. G-292 cells were stimulated by LIPUS for 20 min/day. G-292 cells were seeded on pre-wetted scaffolds by surface injection. Cell growth was analyzed by Alamar Blue and MTT assay. Results LIPUS enhanced cell proliferation inside the static bioreactor by 2.4-fold (day 3) and 1.8-fold (day 7), and the perfusion bioreactor by 2.2-fold (day 3) and 2.4-fold (day 7). The results suggested that LIPUS stimulation has been more effective in static condition than perfusion condition. ConclusionIn conclusion, using single physical signals as stimulation factor has more effective than using different loading on cancerous bone cells together. It’s related to the molecular pathway into the cells for each physical signal that is individual and also cell behavior. Cell behavior and cell response to each treatment could be characterized when each treatment used alone in bone tissue engineering

کلیدواژه ها:

Fluid shear stress ، Low intensity pulsed ultrasound ، Cell behavior ، Bone tissue engineering.